The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.

ROTI^®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.

ROTI^®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI^®Load DNA. ROTI^®Load DNA gel loading buffer is usually used as 1x concentrated solution.

In order to cover the main size reange needed in gel electrophoresis, ROTI^®Load DNA (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image).

Glycerin is the standard reagent for increasing sample density.

Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.