ROTI^®Dex Medium is a spherical, porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.

In gel filtration, also called size exclusion chromatography, molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.

ROTI^®Dex enables a form of group separation for the separation of undesired, low-molecular-weight factors such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.

ROTI^®Dex-50 medium is excellent for the separation of proteins >25 kDa and nucleic acids >20 bp. Purified biomolecules are not significantly diluted when processed with ROTI^®Dex-50 medium.

Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.