Recombinant Mouse OX40/TNFRSF4 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM041200-10, PKSM041200-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: OX40; TNFRSF4

Target Symptom: Tumor necrosis factor receptor superfamily member 4; Tnfrsf4; OX40; CD134; Txgp1

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-6His

UNIProt ID: P47741

Accession: P47741

Background: OX40, also termed CD134 and TNFRSF4, is a T cell co-stimulatory molecule of the TNF receptor superfamily which plays a key role in the survival and homeostasis of effector and memory T cells. OX40 is expressed on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. The interaction between OX40 and OX40 ligand (OX40L) will occur when activated T cells bind to professional antigen-presenting cells (APCs). The T-cell functions, including cytokine production, expansion, and survival, are then enhanced by the OX40 costimulatory signals. OX40 signals are critical for controlling the function and differentiation of Foxp3+ regulatory T cells. OX40-OX40L interaction regulates T-cell tolerance, peripheral T-cell homeostasis, and T-cell-mediated inflammatory diseases.

Activity: Immobilized Mouse OX40L-His(Cat: PKSM041119) at 0.8μg/ml(100 μl/well) can bind Biotinylated Mouse OX40-His. The ED50 of Mouse OX40-His is 23.17 ug/ml.

Sequence: Val20-Pro211

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 22.1 kDa

Observed MW: 40 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only