Sucrase Activity Assay Kit

Size: 96T

Catalogue Number: E-BC-K751-M-96

Citations, Manuals and MSDS Available upon request.

Research Areas: Inorganic Ions;Plant Physiology Series;Inorganic Ions

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: animal tissu

Assay Time: 70 min

Detection Type: Colorimetric method

Sensitivity: 20 U/mL

Detection Range: 20-2000 U/mL

Test Principle: Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.

Inter CV: 6.5

Intra CV: 5.4

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag